Therefore, Fe/sub 2/O/sub 3/ can enhance the metabolic activation of BaP by the lung, as well as act as a carrier for penetration and retention of BaP in the lung. These data suggest that pulmonary exposure to a known cocarcinogen, Fe/sub 2/O/sub 3/, in the presence of BaP results in increased production of dihydrodiols of BaP, which may be further metabolized to the ultimate carcinogenic form(s) of BaP.
Fe/sub 2/O/sub 3/ pretreatment to the whole animal resulted in an increase in the total rate of appearance of metabolites of BaP in the blood, while Fe/sub 2/O/sub 3/ administration to the IPL resulted in a decrease in the total rate of appearance of BaP metabolites in the blood and inhibited the effect of pretreatment. Fe/sub 2/O/sub 3/ pretreatment to the whole animal or administration of Fe/sub 2/O/sub 3/ to the IPL altered BaP metabolism by the perfused lung. Patterns of BaP metabolites were determined by chromatographic techniques and liquid scintillation counting. BaP and its metabolites were also isolated from lung tissue, washout fluid, macrophage, and trachea bronchi at the end of the perfusion at 180 min.
Partition coefficient calculation tool serial#
BaP and its metabolites were isolated from serial blood samples up more » to 180 min after administration of (/sup 14/C)BaP to the IPL. (/sup 14/C)-BaP was administered intratracheally to an isolated perfused lung (IPL) preparation with and without Fe/sub 2/O/sub 3/ after intraperitoneal pretreatment of the whole animal with BaP or intratracheal pretreatment of the whole animal with Fe/sub 2/O/sub 3/ and/or BaP. To facilitate calculation of partition coefficients at other temperatures, we also determined the correlation between temperature and anesthetic solubility, which allowed derivation of empirical equations to calculate partition coefficients for each anesthetic at temperatures between 4 degree signC and 37 degree signC.
« lessĪn isolated perfused New Zealand rabbit lung preparation was used to investigate the effects of a cocarcinogen, ferric oxide (Fe/sub 2/O/sub 3/), on the metabolism of benzo(a)pyrene (BaP), a ubiquitous potent carcinogen that has been associated with the increased incidence of human bronchiogenic carcinoma in occupational and urban settings. = for PCNs show a stepwise change and increase by more than 3 orders of magnitude from the 2-Cl to 6-Cl homologue groups.
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